Our science


Complement Effectors Of Inflammation In Cystic Fibrosis Lung Fluid Correlate With Clinical Measures Of Disease

(8) PLoS One. 2015 Dec 7;10(12):e0144723. doi: 10.1371/journal.pone.0144723. eCollection 2015.


In cystic fibrosis (CF), lung damage is mediated by a cycle of obstruction, infection, and inflammation. Here we explored complement inflammatory effectors in CF lung fluid. In this study soluble fractions (sols) from sputum samples of 15 CF patients were assayed for complement effectors and analyzed with clinical measurements. The pro-inflammatory peptide C5a was increased 4.8-fold (P = 0.04) in CF sols compared with controls. Incubation of CF sols with P. aeruginosa or S. aureus increased C5a concentration 2.3-fold (P = 0.02). A peptide inhibitor of complement C1 (PIC1) completely blocked the increase in C5a concentration from P. aeruginosa in CF sol in vitro (P = 0.001). C5a concentration in CF sol correlated inversely with body mass index (BMI) percentile in children (r = -0.77, P = 0.04). C3a, which has anti-inflammatory effects, correlated positively with FEV1% predicted (rs = 0.63, P = 0.02). These results suggest that complement effectors may significantly impact inflammation in CF lung fluid.

PMID: 26642048 PMCID: PMC4671727 DOI: 10.1371/journal.pone.0144723

More Abstracts

Aug 20, 2020

Incompatible Erythrocyte Transfusion With Lipopolysaccharide Induces Acute Lung Injury...

Acute transfusion reactions can manifest in many forms including acute hemolytic transfusion reaction, allergic reaction and transfusion-related...

Read More
Mar 28, 2018

Inhibition of Immune Complex Complement Activation And Neutrophil Extracellular...

Abstract: Two major aspects of systemic lupus erythematosus (SLE) pathogenesis that have yet to be targeted therapeutically are immune complex-i...

Read More
Dec 31, 2019

Inhibition of Complement Activation, Myeloperoxidase, NET Formation And Oxidant...

A product of rational molecular design, PA-dPEG24 is the lead derivative of the PIC1 family of peptides with multiple functional abilities inclu...

Read More